The progress of research on the influencing factor of nuclear transfer / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 895-899, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-294545
ABSTRACT
Nuclear transfer (NT) is a new cloning technology developed in recent years. NT methods consist of electrofusion, NT mediated by polyethylene glycol (PEG) and microinjection. The success of somatic nuclear transfer depends on the source of donor nucleus, developmental stage of recipient cytoplasts, cell cycle synchrony of donor nucleus. Different methods of harvesting cells have effect on the efficiency of NT. The somatic nucleus will be reprogrammed after NT and will restore a totipotent state in order to undergo development.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oócitos
/
Fisiologia
/
Diferenciação Celular
/
Divisão Celular
/
Células Cultivadas
/
Clonagem de Organismos
/
Biologia Celular
/
Transferência Embrionária
/
Técnicas de Transferência Nuclear
/
Reprogramação Celular
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2009
Tipo de documento:
Artigo
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