Cloning and expression of extracellular region gene located in N-terminus of Leishmania Donovani / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 820-824, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-294561
ABSTRACT
The objective of this study was to construct and express recombinant prokaryotic plasmid pET32a (+)- ast1 in E. coli BL21(DE3). Amastin gene was amplified from genomic DNA of Leishmania Donovani and its transmembran region was predicted by the methods of SOSUI and Tmpred; astl located in N-terminus of amastin gene was amplified and cloned into prokaryotic plasmid pET32a(+), which was named pET32a(+)-ast1, and then rAST1 was expressed in E. coli BL21(DE3). The results of SDS-PAGE and immunobloting assay showed that a fusion protein rAST1 (relative molecular mass about 27 kDa) was able to express in BL21. The recombinant prokaryotic plasmid pET32a(+)- ast1 was successfully constructed, and noted to be efficiently expressed in E. coli BL21(DE3).
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Leishmania donovani
/
Proteínas Recombinantes de Fusão
/
Proteínas de Protozoários
/
Clonagem Molecular
/
Genes de Protozoários
/
Escherichia coli
/
Espaço Extracelular
/
Genética
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2009
Tipo de documento:
Artigo
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