Up-regulation of Fas is related to apoptosis of HeLa cells induced by co-immobilized TNF-alpha/IFN-gamma / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 615-619, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-294606
ABSTRACT
This study was aimed to examine the expression of apoptosis-associated gene Fas in HeLa cell, explore the effects of the co-immobilized cytokines (tumor necrosis factor-alpha and interferon-gamma), and probe the potential mechanism of action. The preparation and application of the research couple IFN-gamma and TNF-alpha to the polystyrene cell culture plate were performed using the Photo-immobilization method, with different doses (20 ng/well and 200 ng/well) and synthesized optical active material. HeLa cells were treated with cytokines for two dose and 1, 3, 6 days. The result showed that the free cytokines induced HeLa apoptosis quickly, yet the HeLa apoptosis induced by co-immobilized cytokines had longer effect.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Células HeLa
/
Regulação para Cima
/
Química
/
Interferon gama
/
Fator de Necrose Tumoral alfa
/
Apoptose
/
Receptor fas
/
Sinergismo Farmacológico
/
Proteínas Imobilizadas
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2009
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS