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TIMP-1 secreted by fibroblasts inhibits tumor cell invasion and metastasis in mouse melanoma / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 610-614, 2009.
Artigo em Chinês | WPRIM | ID: wpr-294607
ABSTRACT
We constructed a recombinant adenoviral vector expressing human tissue inhibitors of metalloproteinase-1(TIMP-1), and evaluated the inhibition of TIMP-1 secreted by primary fibroblasts after infection with adenovirus-mediated TIMP-1 gene (Ad-TIMP-1) on tumor cell invasion and metastasis in mouse melanoma. It was found that TIMP-1 was detected in the supernatants of cultured mouse primary fibroblasts after infection with Ad-TIMP-1 by indirect enzyme-linked immunosorbent assay (ELISA). The TIMP-1 secreted by Ad-TIMP-1 infected primary fibroblast significantly inhibited B16BL6 cell invasion and metastasis both in vitro and in vivo. We also demonstrated that the primary fibroblasts transfected by Ad-TIMP-1, after being subcutaneously injected into mouse, can secreted TIMP-1 into the blood of mouse and maintained at the therapeutic in vivo levels of TIMP-1. These results suggest that the preparation of Ad-TIMP-1 infected primary fibroblast be an effective method to deliver TIMP-1 gene in vivo, which provides a new strategy of gene therapy and has the potential for clinical applications in the treatment of tumor cell metastasis.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Terapêutica / Melanoma Experimental / Proteínas Recombinantes / Terapia Genética / Adenoviridae / Inibidor Tecidual de Metaloproteinase-1 / Fibroblastos / Genética Limite: Animais / Feminino / Humanos Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2009 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Terapêutica / Melanoma Experimental / Proteínas Recombinantes / Terapia Genética / Adenoviridae / Inibidor Tecidual de Metaloproteinase-1 / Fibroblastos / Genética Limite: Animais / Feminino / Humanos Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2009 Tipo de documento: Artigo