Effect of lipopolysaccharide on the stromal cell-derived factor-1 expression in human stem cells from apical papilla / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 346-351, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-294705
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of stromal cell-derived factor-1 (SDF-1) in human stem cells from apical papilla (SCAP), and to evaluate the effect of lipopolysaccharide (LPS) on SDF-1 expression by SCAP.</p><p><b>METHODS</b>SCAP were isolated from dental papilla of human immature third molars. The expression of SDF-1 was evaluated by reverse transcription-PCR (RT-PCR). After SCAP being exposed to different concentrations (0.1, 1.0, 10 mg/L) of LPS for 24 and 48 h, the effect of LPS on cell proliferation and gene expression of SDF-1 was investigated by cell counting kit-8 and real-time PCR respectively, while cells without LPS stimulation were considered as negative control.</p><p><b>RESULTS</b>LPS had no significant effect on SCAP proliferation until day 7. RT-PCR assays demonstrated that SCAP expressed SDF-1 mRNA. Different concentrations of LPS significantly promoted the SDF-1 expression in SCAP after 24 h (F = 12.102, P = 0.002) and 48 h (F = 39.054, P < 0.001) exposure, with relative gene expression ratio (experimental/control) increased to 1.4 ± 0.1, 2.2 ± 0.4, 2.3 ± 0.5 in 24 h group and 2.1 ± 0.4, 3.4 ± 0.3, 3.8 ± 0.5 in 48 h group.</p><p><b>CONCLUSIONS</b>Isolated SCAP in cultures have the expression of SDF-1 mRNA. LPS can significantly promote the expression of SDF-1 in SCAP.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Células-Tronco
/
Expressão Gênica
/
Diferenciação Celular
/
Lipopolissacarídeos
/
Biologia Celular
/
Papila Dentária
/
Proliferação de Células
/
Quimiocina CXCL12
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2015
Tipo de documento:
Artigo
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