Identification of differentially expressed genes in myocardium of patients with heart failure by human whole genomic oligonucleotide microarray-assisted pathways analysis / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 120-125, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-294766
ABSTRACT
<p><b>OBJECTIVE</b>To identify the differentially expressed gene profiles in myocardium of patients with heart failure using human whole genomic oligonucleotide microarray-assisted pathway analysis.</p><p><b>METHODS</b>Phalanx whole genomic oligonucleotide microarrays were used to detect the gene expression profiles of myocardium in four patients died of heart failure and 4 brain died patients without heart diseases. The microarray findings were confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The genes with a threshold of 1.2 times fold-change were selected and BioCarta Pathway and KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathway databases were used to identify functionally related gene pathways.</p><p><b>RESULTS</b>A total of 2806 genes with differentially expression were detected between the failing and non-failing heart samples, expression changes of 399 genes were more than 2-folds. Eleven pathways were identified by BioCarta pathway database and sixteen pathways were identified by KEGG PATHWAY Database.</p><p><b>CONCLUSION</b>Genomic microarray-assisted pathway analysis could help to identify gene expression profiles in failing heart.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA
/
Transdução de Sinais
/
Genoma Humano
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Análise de Sequência com Séries de Oligonucleotídeos
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Perfilação da Expressão Gênica
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Estudo de Associação Genômica Ampla
/
Genes
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Genética
/
Genótipo
/
Insuficiência Cardíaca
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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