Resveratrol inhibits EGF-induced invasion of human lung adenocarcinoma A549 cells / 中国肺癌杂志
Chinese Journal of Lung Cancer
; (12): 287-291, 2010.
Article
em Zh
| WPRIM
| ID: wpr-294820
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND AND OBJECTIVE</b>Invasion and metastasis are the primary causes of death in patients with pulmonary carcinoma. The epidermal growth factor (EGF) stimulates A549 cells invasion greatly through activating ERK and PI3K-Akt signaling pathway. The aim of this study is to elucidate the inhibitory effect of Resveratrol on EGF-induced invasive ability of A549 cells in vitro and explore the molecular mechanism.</p><p><b>METHODS</b>The cytotoxicity of Resveratrol was evaluated by methyl thiazolyltetrazolium (MTT) assay. Then, the A549 cells were treated with EGF and non-cytotoxic concentration of Resveratrol. The cells' invasion were detected by Boyden chamber assay; MMP-2 activity was determined by gelatine zymography assay; the changes of the related proteins were detected by Western blot.</p><p><b>RESULTS</b>Resveratrol was not toxic to A549 cells at the concentration between 0 to 30 microM. The invasion ability of EGF-induced A549 cells was decreased after treatment with 20 microM resveratrol for 24 h, accompanied by the inhibition of MMP-2 secretion. And the levels of p-ERK1/2, PI3K (within 6 h) were suppressed too.</p><p><b>CONCLUSION</b>20 microM Resveratrol inhibits A549 cells' invasion possibly through the suppression of the activation of ERK and PI3K-Akt signaling pathways, subsequently exerting inhibitory effect on MMP-2.</p>
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Assunto principal:
Farmacologia
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Estilbenos
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Adenocarcinoma
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Transdução de Sinais
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Western Blotting
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Anticarcinógenos
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Fosfatidilinositol 3-Quinases
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Proteína Quinase 1 Ativada por Mitógeno
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Linhagem Celular Tumoral
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Proteína Quinase 3 Ativada por Mitógeno
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Lung Cancer
Ano de publicação:
2010
Tipo de documento:
Article