Effect of Salvia miltiorrhiza injection on hyperpolarization-activated current channels in dorsal root ganglion neurons of rats / 药学学报

ABSTRACT

<p><b>AIM</b>To explore the modulation of Salvia miltiorrhiza on hyperpolarization-activated current (Ih) channels in dorsal root ganglion (DRG) neurons of rats and identify the mechanism of Salvia miltiorrhiza in alleviating pain and inhibiting calcium overload. Methods The effect of Salvia miltiorrhiza injection on Ih channels in DRG neurons of rats were examined by using whole-cell patch clamp technique. Results The experimental results showed that the amplitude of Ih evoked by -150 mV was (-1.06 +/- 0.18) nA. The Ih could be fitted well into the single kinetics and the time constant of activation, pi was clearly voltage-dependent with tau = (322.14 +/- 28.81) ms at -100 mV, decreasing to tau = (62.51 +/- 9.78) ms at -150 mV. The reversal potential of Ih was (-35.03 +/- 1.12) mV measured from tail currents. But no significant differences were found between the DRG neurons in the absence and presence of Salvia miltiorrhiza injection (10%, 25%, 50%) in the current amplitude, the time constant of activation and the reversal potential. The only difference between the DRG neurons in the absence and presence of Salvia miltiorrhiza injection was the half-activation potential of Ih. In control recordings the half-activation potential was (-106.07 +/- 3.59) mV. By comparison, the half-activation potentials changed to (-111.59 +/- 3.79) mV (n=31 neurons, P < 0.05), (-119.37 +/- 4.96) mV (n=31 neurons, P < 0.05) and (-121.23 +/- 3.86) mV (n=31 neurons, P < 0.05) in the presence of 10%, 25%, 50% Salvia miltiorrhiza injection, respectively.</p><p><b>CONCLUSION</b>Only the half-activation potential of Ih in the arthritic and neuropathic rat models shifted in the depolarizing direction, which increased the electrophysiological activity of Ih and made it related to peripheral hyperalgesia. The selective inhibition of Salvia miltiorrhiza on the electrophysiological activity of Ih may be one of the mechanisms underlying its analgesic effects.</p>