The roles of c-Jun and CBP in the inhibitory effect of quercetin on prostate cancer cells / 药学学报
Acta Pharmaceutica Sinica
;
(12): 819-824, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-294932
ABSTRACT
<p><b>AIM</b>To further uncover the possible mechanism of quercetin-mediated inhibitory effect on prostate cancer cells.</p><p><b>METHODS</b>The cell extracts treated with quercetin or without treatment were used for checking protein expression levels of c-Jun and cAMP response element binding protein (CREB)-binding protein (CBP) by Western blotting assay. Regulatory effects of c-Jun and CBP on the function of androgen receptor (AR) were examined by cotransfection experiment. Finally, a physical interaction of c-Jun and the AR was investigated by coimmunoprecipitation.</p><p><b>RESULTS</b>Quercetin dramatically induced the protein expression of c-Jun which in turn inhibited the AR function. Meanwhile, quercetin had no detectable effect on CBP expression, and the results of transient transfection demonstrated that the ectopic CBP stimulated the transcriptional activity of AR, whereas CBP-mediated stimulation could be attenuated by quercetin. Furthermore, physical interaction of c-Jun and the AR was confirmed by coimmunoprecipitation result.</p><p><b>CONCLUSION</b>Overexpression of c-Jun induced by quercetin had inhibitory effect on the function of AR protein, and increased CBP expression did not reverse the inhibition by quercetin. Together, quercetin-mediated inhibition on the AR function might be not by competition with limited amount of CBP in the cell, but through a direct association of c-Jun and the AR.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Fisiologia
/
Neoplasias da Próstata
/
Ligação Proteica
/
Quercetina
/
Transfecção
/
Receptores Androgênicos
/
Proteínas Proto-Oncogênicas c-jun
/
Linhagem Celular Tumoral
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2006
Tipo de documento:
Artigo
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