RP-HPLC fingerprint for quality assessment of Radix Angelicae dahuricae / 药学学报

ABSTRACT

<p><b>AIM</b>To establish the RP-HPLC fingerprint analysis for the quality control of Radix Angelicae dahuricae.</p><p><b>METHODS</b>HPLC fingerprint analysis method of Radix Angelicae dahuricae was developed. Kromasil C18 column (250 mm x 4.6 mm ID, 5 microm) was used, with mixture of acetonitrile and water as mobile phase in a gradient mode. The flow rate was 1.0 mL x min(-1). The wavelength of measurement was 254 nm. Twenty-one batches of Radix Angelicae dahuricae were determined.</p><p><b>RESULTS</b>The 21 samples were classified as 4 clusters by cluster analysis and the 11 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004.</p><p><b>CONCLUSION</b>The method can be used to identify and evaluate the quality of Radix Angelicae dahuricae conveniently.</p>