Heme oxygenase-1 and carbon monoxide are key mediators for vascular smooth muscle cells proliferation induced by insulin-like growth factor-I / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 153-158, 2006.
Article
em Zh
| WPRIM
| ID: wpr-295355
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the role and related mechanisms of heme oxygenase-1/carbon monoxide (HO-1/CO) on VSMCs proliferation induced by insulin-like growth factor-I (IGF-I).</p><p><b>METHODS</b>VSMCs isolated from rabbit aorta were cultured in vitro and proliferation was induced by IGF-I. Hemin (a substrate and inducer of HO-1) or zinc protoporphyrin-IX (Znpp-IX, an inhibitor of HO-1) was added to stimulate or inhibit the expression of HO-1. The mRNA and protein expressions of HO-1 were detected by RT-PCR and Western blot analysis. CO released into the culture media was quantitated by measuring carbon monoxide hemoglobin (COHb), VSMCs proliferation and cell cycle were determined by (3)H-TdR incorporation assay and flow cytometry, respectively.</p><p><b>RESULTS</b>The HO-1 mRNA and protein expressions in VSMCs and the amount of COHb in the culture media were significantly increased and the IGF-I-induced (3)H-TdR incorporations of VSMCs significantly reduced by hemin in a dose-dependent manner (P < 0.01). Furthermore, VSMCs in the G(0)/G(1) phase were increased and in the S and G(2)/M phase decreased by hemin (P < 0.01). Opposite results were observed in VSMCs treated with Znpp-IX.</p><p><b>CONCLUSIONS</b>Endogenous HO-1 and CO are important mediators for inhibiting IGF-I induced VSMCs proliferation by reducing VSMCs DNA synthesis and decelerating cell cycle progression.</p>
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Índice:
WPRIM
Assunto principal:
Farmacologia
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RNA Mensageiro
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Fator de Crescimento Insulin-Like I
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Monóxido de Carbono
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Células Cultivadas
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Biologia Celular
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Miócitos de Músculo Liso
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Proliferação de Células
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Heme Oxigenase-1
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Genética
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2006
Tipo de documento:
Article