Studies on survival and outgrowth of processes of cultured rat hippocampus neurons in containing selenium and free serum medium / 中华预防医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of selenium on cultured newborn rat's hippocampus neurons survival and outgrowth development.</p><p><b>METHODS</b>Using the technique of primary culture of hippocampal neurons of newborn rat. The different dose of Se (62.5 microgram/L, 125.0 microgram/L, 182.5 microgram/L) were added into the medium at same time. We not only investigated the number of survival of neurons on 1 - 14 d and 1d to 10 d in with and without serum containing-Se medium, but also observed the length outgrowth of the neurite at 16 h, 24 h, 36 h, 48 h during culture.</p><p><b>RESULTS</b>Selenium could obviously enhance the outgrowth of early processed in 10% fetal serum medium and average length of neurite outgrowth is 15 - 20 micrometer more longer than control groups (P < 0.01) and selenium could also increase the livability of neurons and prolong survival time of cultured neurons in serum-free medium.</p><p><b>CONCLUSIONS</b>Selenium may play a very important role for early processed growth and development of hippocampal neurons.</p>