Application of SCGE-FPG in the study of arsenic-induced oxidative DNA damage in PHA-stimulated and unstimulated human lymphocytes / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 12-15, 2002.
Article
em Zh
| WPRIM
| ID: wpr-295793
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To confirm that arsenic (As) induces oxidative DNA damage in phytohemagglutinin (PHA)-stimulated and unstimulated human lymphocytes.</p><p><b>METHODS</b>The alkaline comet assay combined with specific enzyme (Formamidopyrimidine-DNA glycosylase, FPG) digestion was used to measure As-induced base damage.</p><p><b>RESULTS</b>The enzyme-sensitive sites were readily detected with the alkaline comet assay after the cells were treated with 10 micromol As for 2 hours. The repair patterns observed for FPG-created DNA single strand breaks (SSBs) in As-treated cells were comparable to those in hydrogen peroxide (H(2)O(2))-treated cells. The enzyme-created SSBs, As-induced base damage, were more significantly revealed in PHA-stimulated lymphocytes. About 63% and 68% of SSBs induced by As and H(2)O(2), respectively, were repaired in PHA-stimulated lymphocytes by 2-hour repair incubation, but about 34% and 43%, respectively, were repaired in unstimulated cells. About 40% and 49% of base damage induced by As and H(2)O(2), respectively, were repaired in PHA-stimulated lymphocytes, but about 19% and 21 %, respectively, were repaired in unstimulated cells.</p><p><b>CONCLUSIONS</b>As induces oxidative DNA damage in human lymphocytes within micromolar concentrations. Like the damage induced by H(2)O(2), As-induced DNA damage was more slowly repaired in unstimulated lymphocytes.</p>
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Índice:
WPRIM
Assunto principal:
Oxirredução
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Farmacologia
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Fito-Hemaglutininas
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Arsênio
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Dano ao DNA
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DNA de Cadeia Simples
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Linfócitos
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DNA-Formamidopirimidina Glicosilase
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Reparo do DNA
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Eletroforese
Limite:
Adult
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2002
Tipo de documento:
Article