Application of mesenchymal stem cells as a vehicle to deliver replication-competent adenovirus for treating malignant glioma / 癌症
Chinese Journal of Cancer
;
(12): 233-240, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-295890
ABSTRACT
Although gene therapy was regarded as a promising approach for glioma treatment, its therapeutic efficacy was often disappointing because of the lack of efficient drug delivery systems. Mesenchymal stem cells(MSCs) have been reported to have a tropism for brain tumors and thus could be used as delivery vehicles for glioma therapy. Therefore, in this study, we attempted to treat glioma by using MSCs as a vehicle for delivering replication-competent adenovirus. We firstly compared the infectivity of type 3, type 5, and type 35 fiber-modified adenoviruses in MSCs. We also determined suitable adenovirus titer in vitro and then used this titer to analyze the ability of MSCs to deliver replication-competent adenovirus into glioma in vivo. Our results indicated that type 35 fiber-modified adenovirus showed higher infectivity than did naked type 3 or type 5 fiber-modified adenovirus. MSCs carrying replication-competent adenovirus significantly inhibited tumor growth in vivo compared with other control groups. In conclusion, MSCs are an effective vehicle that can successfully transport replication-competent adenovirus into glioma, making it a potential therapeutic strategy for treating malignant glioma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Terapêutica
/
Replicação Viral
/
Neoplasias Encefálicas
/
Distribuição Aleatória
/
Adenoviridae
/
Ensaios Antitumorais Modelo de Xenoenxerto
/
Linhagem Celular Tumoral
/
Transplante de Células-Tronco Mesenquimais
/
Terapia Viral Oncolítica
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Chinese Journal of Cancer
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS