Detection and molecular characterization of enteroviruses in Korean surface water by using integrated cell culture multiplex RT-PCR / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 425-431, 2008.
Article
em En
| WPRIM
| ID: wpr-296029
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify waterborne enteric viruses in Korean surface water.</p><p><b>METHODS</b>Integrated cell culture (ICC)-multiplex reverse transcription-polymerase chain reaction (RT-PCR) was simultaneously designed to detect coxsackieviruses (CV), polioviruses (PV), and reoviruses (RV). ICC-multiplex RT-PCR and phylogenetic analysis were conducted using 21 total culturable virus assay (TCVA)-positive sample-inoculated cell cultures.</p><p><b>RESULTS</b>CV and RV were detected in 9 samples each, and 3 samples were positive for both CV and RV. PV was not detected in any sample. Molecular phylogenetic analysis of the VP1 gene sequences revealed that CV types B2 and B4 predominated in Korean surface water, and the nucleotide sequences of CV type B2 were clustered with those of CVs isolated from China and Japan. The results suggested that the evolution of these viruses occurred in a region-specific manner.</p><p><b>CONCLUSION</b>CV and RV are detectable in Korean surface water, with a predominance of CV type B2, and the evolution of CV type B2 occur in a region-specific manner.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Filogenia
/
Virologia
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Microbiologia da Água
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Abastecimento de Água
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Células Cultivadas
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Classificação
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Enterovirus
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Água Doce
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Genética
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2008
Tipo de documento:
Article