Breast cancer resistance protein expression and 5-fluorouracil resistance / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 290-295, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-296049
ABSTRACT
<p><b>OBJECTIVE</b>To filtrate breast cancer resistance protein (BCRP)-mediated resistant agents and to investigate clinical relationship between BCRP expression and drug resistance.</p><p><b>METHODS</b>MTT assay was performed to filtrate BCRP-mediated resistant agents with BCRP expression cell model and to detect chemosensitivity of breast cancer tissue specimens to these agents. A high performance liquid chromatography (HPLC) assay was established, and was used to measure the relative dose of intracellular retention resistant agents. RT-PCR and immunohistochemistry (IHC) were employed to investigate the BCRP expression in breast cancer tissue specimens.</p><p><b>RESULTS</b>MTT assay showed that the expression of BCRP increased with the increasing resistance of 5-fluorouracil (5-Fu) (P<0.05, n=3) in the cell model, while HPLC assay indicated that the intracellular retention dose of 5-Fu was significantly correlated with the expression of BCRP (r=-0.897, P<0.05, n=3). A total of 140 breast cancer tissue specimens were collected. BCRP-positive expression was detected in forty-seven specimens by both RT-PCR and IHC. As shown by MTT assay subsequently, the resistance index (RI) of 47 BCRP-positive breast cancer tissue specimens to 5-Fu was 7-12 times as high as that of adjacent normal tissue samples. BCRP expression was related to 5-Fu resistance (R2=0.8124, P<0.01).</p><p><b>CONCLUSION</b>Resistance to 5-Fu can be mediated by BCRP. Clinical chemotherapy for breast cancer patients can be optimized based on BCRP-positive expression.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Imuno-Histoquímica
/
Cromatografia Líquida de Alta Pressão
/
Transportadores de Cassetes de Ligação de ATP
/
Resistencia a Medicamentos Antineoplásicos
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Fluoruracila
/
Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP
/
Metabolismo
/
Proteínas de Neoplasias
Limite:
Adulto
/
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2008
Tipo de documento:
Artigo
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