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Effect of Deletion of the Carboxyl Terminal of the NS1 Protein on Pathogenicity of the Influenza B Virus / 病毒学报
Chinese Journal of Virology ; (6): 404-409, 2015.
Artigo em Chinês | WPRIM | ID: wpr-296270
ABSTRACT
To analyze the molecular basis of the variation of the pathogenicity of the influenza B virus, we rescued a recombinant virus with a deletion in the carboxyl terminal of the NS1 protein using reverse genetics based on the parental virus B-S9 of B/Yamagata/16/88. A mutant strain with a deletion of 171 amino acids in the carboxyl terminal of the NS1 protein was named "B-L5". BALB/c mice were inoculated with 3 X 105 EID50 of B-L5 and the parental virus B-S9, respectively. Then, weight changes, survival, and viral titers were documented. During 3 days post-inoculation (dpi) to 7 dpi, the weight of mice infected with B-S9 decreased. However, the weight of mice infected with B-L5 showed weight decreases only at 2 dpi, and quickly recovered at 3 dpi. B-S9 and B-L5 could replicate in the lungs of BALB/c mice. However, viral titers in the lungs of mice infected with B-L5 were 7900-times lower than those of mice infected with B-S9 at 3 dpi. Viral titers in the lungs of mice infected with B-L5 were not detected at 6 dpi. These results showed that, compared with the parent virus B-S9, the mutant virus B-L5 showed lower pathogenicity in BALB/c mice. Our study suggests that deletion of the carboxyl terminal of the NS1 protein decreases the pathogenicity of the influenza B virus. Establishment of a reverse-genetics system for the B influenza virus will provide a platform for studying its pathogenesis, and mechanism of transmission, and for developing live-attenuated influenza B virus vaccines.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Vírus da Influenza B / Fisiologia / Virulência / Peso Corporal / Análise de Sobrevida / Química / Proteínas não Estruturais Virais / Deleção de Sequência / Carga Viral / Células HEK293 Limite: Animais / Feminino / Humanos Idioma: Chinês Revista: Chinese Journal of Virology Ano de publicação: 2015 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Vírus da Influenza B / Fisiologia / Virulência / Peso Corporal / Análise de Sobrevida / Química / Proteínas não Estruturais Virais / Deleção de Sequência / Carga Viral / Células HEK293 Limite: Animais / Feminino / Humanos Idioma: Chinês Revista: Chinese Journal of Virology Ano de publicação: 2015 Tipo de documento: Artigo