Characterization and Expression Analysis of Peroxiredoxin Genes in NNK-induced V79 Cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 224-228, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-296493
ABSTRACT
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a potent and prevalent nitrosamine procarcinogen found in cigarette smoke. The aim of this work is to study alterations in peroxiredoxin (Prx) expression induced by NNK during carcinogenesis. Characterization of Prx genes from hamster was performed using bioinformatics. V79 cells were induced with different concentrations of NNK (0.1-0.4 mg/mL), and the expression levels of six Prx genes (Prx1-Prx6) were measured by qRT-PCR 24 h following NNK treatment. Prx gene expression was induced by NNK stress, and the highest transcription levels were induced by over 20.42-fold relative to that of the control. NNK induced alterations in Prx expression over the course of lung cancer, which means Prxs may play important roles in ROS detoxification under NNK stress and their functions are complementary.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Carcinógenos
/
Linhagem Celular
/
Sobrevivência Celular
/
Regulação da Expressão Gênica
/
Cricetulus
/
Relação Dose-Resposta a Droga
/
Toxicidade
/
Peroxirredoxinas
/
Genética
/
Metabolismo
Limite:
Animais
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2017
Tipo de documento:
Artigo
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