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Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 594-598, 2016.
Artigo em Inglês | WPRIM | ID: wpr-296563
ABSTRACT
We used Smo siRNA to inhibit hedgehog signaling pathway in embryonic day (E) 13 palatal shelves in organ culture. SiRNA 4 was chosen as the most efficient from four synthesized Smo siRNAs. Palatal shelf fusion rate of 4 μg/mL cyclopamine group was the lowest and significantly lower than that of blank control group (P<0.05), and that of siRNA 4 group was also lower than that of blank control group (P=0.183). At 48 h after transfection, Smo protein level of siRNA 4 group was 64.8% lower than that of blank control group (P<0.05), and Gli1 protein level of 4 μg/mL cyclopamine group was 68.9% lower than that of blank control group (P<0.05). Hedgehog signaling pathway inhibition decreased palatal fusion in organ culture, probably owing to downregulation of Smo and Gli1 proteins.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Palato / Transdução de Sinais / Embriologia / RNA Interferente Pequeno / Fatores de Transcrição Kruppel-Like / Proteínas Hedgehog / Proteína Gli3 com Dedos de Zinco / Proteína Gli2 com Dedos de Zinco / Genética / Metabolismo Limite: Animais Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2016 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Palato / Transdução de Sinais / Embriologia / RNA Interferente Pequeno / Fatores de Transcrição Kruppel-Like / Proteínas Hedgehog / Proteína Gli3 com Dedos de Zinco / Proteína Gli2 com Dedos de Zinco / Genética / Metabolismo Limite: Animais Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2016 Tipo de documento: Artigo