Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 594-598, 2016.
Article
em En
| WPRIM
| ID: wpr-296563
Biblioteca responsável:
WPRO
ABSTRACT
We used Smo siRNA to inhibit hedgehog signaling pathway in embryonic day (E) 13 palatal shelves in organ culture. SiRNA 4 was chosen as the most efficient from four synthesized Smo siRNAs. Palatal shelf fusion rate of 4 μg/mL cyclopamine group was the lowest and significantly lower than that of blank control group (P<0.05), and that of siRNA 4 group was also lower than that of blank control group (P=0.183). At 48 h after transfection, Smo protein level of siRNA 4 group was 64.8% lower than that of blank control group (P<0.05), and Gli1 protein level of 4 μg/mL cyclopamine group was 68.9% lower than that of blank control group (P<0.05). Hedgehog signaling pathway inhibition decreased palatal fusion in organ culture, probably owing to downregulation of Smo and Gli1 proteins.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Palato
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Transdução de Sinais
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Embriologia
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RNA Interferente Pequeno
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Fatores de Transcrição Kruppel-Like
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Proteínas Hedgehog
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Proteína Gli3 com Dedos de Zinco
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Proteína Gli2 com Dedos de Zinco
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Genética
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Metabolismo
Limite:
Animals
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2016
Tipo de documento:
Article