Cytotoxic Responses and Apoptosis in Rat Kidney Epithelial Cells Exposed to Lead / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 529-533, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-296572
ABSTRACT
The toxic effects of lead on normal rat kidney epithelial cells (NRK cells) may occur via various pathways. However, the role of intrinsic mitochondrial pathway in Lead-induced apoptosis in NRK cells has not been investigated. The purpose of our study was to investigate cytotoxic responses and cell apoptosis mediated by lead in NRK cells. NRK cells were treated with different concentrations of Lead acetate for 12 h to determine the cytotoxicity of lead. Mitochondrial transmembrane potential was also analyzed using a fluorescence spectrophotometer. Moreover, the activities of caspase-3 and caspase-9 were detected in the presence of lead. Finally, the lead-induced cell apoptosis was evaluated by flow cytometry in the present of caspase inhibitors Z-VAD-FMK and Ac-LEHD-FMK, respectively. The results would contribute to clarify the role of Lead in proliferation and apoptosis of NRK cells, and help to understand the underlying mechanism responsible for lead-induced cell apoptosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Compostos Organometálicos
/
Linhagem Celular
/
Sobrevivência Celular
/
Regulação da Expressão Gênica
/
Apoptose
/
Biologia Celular
/
Relação Dose-Resposta a Droga
/
Células Epiteliais
/
Toxicidade
/
Rim
Limite:
Animais
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2016
Tipo de documento:
Artigo
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