Effects of 17beta-estradiol on the intracellular calcium of masticatory muscles myoblast in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 611-613, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-296734
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of 17beta-estradiol on the intracellular calcium of masticatory muscles myoblast.</p><p><b>METHODS</b>Myoblasts from maxillofacial skeletal muscle of one week old female Sprague-Dawley rats were cultured. Fluo-4-AM as the Ca2+ indicator and the laser confocal microscope system were used to observe the effects of estrogen on the cytoplasmic Ca2+ concentration in the normal pH condition and the acid condition (pH = 6.7).</p><p><b>RESULTS</b>In the normal pH condition, when 17beta-estradiol (10(-9), 10(-8), 10(-7) mol/L) were added to cells cytoplasmic Ca2+ immediately increased then decreased right away, and in the end came into a new Ca2+ homeostasis in the base line. In the acid condition, 17beta-estradiol (10(-9), 10(-8), 10(-7) mol/L) made the cytoplasmic Ca2+ decreased immediately then came into a new Ca2+ homeostasis under the base line.</p><p><b>CONCLUSION</b>The results suggest that estrogen may maintain the skeletal cytoplasmic Ca2+ concentration in a lower level and reduce the cytoplasmic Ca2+ accumulation to keep the normal functions of masticatory muscles myoblast.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Xantenos
/
Técnicas In Vitro
/
Cálcio
/
Ratos Sprague-Dawley
/
Mioblastos
/
Estradiol
/
Compostos de Anilina
/
Músculos da Mastigação
Limite:
Animais
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2007
Tipo de documento:
Artigo
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