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Recent advances in enzyme assays using fluoremetry / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1765-1769, 2009.
Artigo em Chinês | WPRIM | ID: wpr-296861
ABSTRACT
Enzymes play such a pivotal role in cellular metabolism that enzyme assays are important for bio-engineering, disease diagnoses and drug discovery. Among the reported methods, fluoremetry has attracted more and more attention due to its high sensitivity and possibility of continuous dynamic monitoring. The recent progresses and applications in enzyme assays using fluorescent probes were reviewed. Different methods were classified into direct fluorescence detection and indirect fluorescence detection according to their labeled substrates and detection mechanisms. Our writing purpose is to provide the readers with a flavor of the kinds of tools and strategies available in enzyme assays with fluorescent probes. Also, the research situation and prospects were disucssed
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Ensaios Enzimáticos / Corantes Fluorescentes / Fluorometria / Métodos / Microscopia de Fluorescência Limite: Animais / Humanos Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2009 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Ensaios Enzimáticos / Corantes Fluorescentes / Fluorometria / Métodos / Microscopia de Fluorescência Limite: Animais / Humanos Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2009 Tipo de documento: Artigo