Preparation and identification of recombinant PTD-maxadilan / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1739-1745, 2009.
Article
em Zh
| WPRIM
| ID: wpr-296864
Biblioteca responsável:
WPRO
ABSTRACT
In order to construct a novel fusion protein PTD-maxadilan (PTD-MAX) that can enter the blood-brain barrier (BBB) efficiently, a new gene encoding PTD-MAX was synthesized and cloned into the expression vector pKYB. The recombinant vector pKYB-PTD-MAX was transformed into Escherichia coli ER2566. The expression of fusion protein consisting of PTD-MAX, intein and chitin binding domain was induced by IPTG and the target PTD-MAX protein was purified using Intein Mediated Purification with an Affinity Chitin-binding Tag system. The molecular weight of PTD-MAX determined by the laser flight mass spectrometry was coherent with its theoretical value. The results of the experiment in vivo indicated that the recombinant PTD-MAX can permeate into BBS and inhibitory effects on the food intake were more significantly than maxadilan (P<0.05). The preparation of PTD-MAX lay the foundation for its further application.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Vasodilatadores
/
Proteínas Recombinantes de Fusão
/
Dados de Sequência Molecular
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Farmacocinética
/
Sequência de Bases
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Barreira Hematoencefálica
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Estrutura Terciária de Proteína
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Proteínas de Insetos
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Escherichia coli
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Vetores Genéticos
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Article