DNA-EGS1386 in cells induced RNase P inhibits the expression of human cytomegalovirus UL49 gene / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1690-1696, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-296871
ABSTRACT
External Guide Sequences (EGSs) represents a novel nucleic acid based gene interference approach to modulate gene expression. They are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. DNA-based EGS1386 with a size of 12 nt was chemically synthesized to target the mRNA coding for the UL49 gene of human cytomegalovirus (HCMV). The DNA-based EGS1386 molecule efficiently directed human RNase P to cleave the target mRNA sequence in vitro. A reduction of more than 50% in the levels of UL49 expression was observed in human cells treated with the DNA-based EGS1386 targeted UL49 assayed by fluorescent quantization PCR and Western blotting. This results showed that the DNA-EGS1386 can effectively guide the RNase P cut the target mRNA. Therefore, DNA-EGS can develop into a new gene silencing technology and potential of the anti-viral reagents.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligodesoxirribonucleotídeos
/
Farmacologia
/
Virologia
/
DNA Viral
/
RNA Mensageiro
/
Sequência de Bases
/
Regulação Viral da Expressão Gênica
/
Proteínas Estruturais Virais
/
Química
/
Infecções por Citomegalovirus
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo
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