Construction and characterization of cardiac specific promoter-driven expression vector / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1546-1551, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-296892
ABSTRACT
We constructed and identified cardiac-specific a-myosin heavy chain (alpha-MHC) promoter-driven expression vector. alpha-MHC promoter was amplified by PCR by using mouse genomic DNA as template and inserted into pGEM-T Easy vector. The inserted fragment was released by enzyme digestion, and then the cytomegalovirus (CMV) promoter in pcDNA3.1(+)-EGFP-hygro vector was replaced by the alpha-MHC promoter to construct alpha-MHC-EGFP expression vector. After identification with enzyme digestion, alpha-MHC-EGFP was transfected into mouse primary cardiomyocytes by electroporation. Green fluorescence could be observed in transfected cardiomyocytes, but not in transfected non-cardiomyocytes. Alpha-MHC-EGFP expression vector was specifically expressed in cardiomyocytes, and could be used to purify embryonic stem cell-derived cardiomyocytes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transfecção
/
Diferenciação Celular
/
Regiões Promotoras Genéticas
/
Eletroporação
/
DNA Complementar
/
Cadeias Pesadas de Miosina
/
Biologia Celular
/
Citomegalovirus
/
Proteínas de Fluorescência Verde
/
Células-Tronco Embrionárias
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo
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