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Recombinant human gapM1 expressed in Pichia pastoris and its anti-diabetic effect / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1211-1217, 2009.
Artigo em Chinês | WPRIM | ID: wpr-296936
ABSTRACT
Adiponectin is an adipokine predominantly synthesized and secreted by adipocytes in the white adipose tissue, and it can lower the blood glucose level and increase free fatty acid oxidation. In the current study, we developed the globular domain of adiponectin (gapM1) to treat type II diabetes. In both flask and fermentor, we cultivated Pichia pastoris expressing recombinant gapM1 and established the purification procedure by using gel filtration and anion exchange chromatography. To evaluate the biological activity of recombinant gapM1, we used rat type II diabetes model fed high-fat diet in combination with low-dose STZ (Streptozocin) induction. We purified 200 mg gapM1 with purity of 96% from 10 liters of supernatant. The recombinant gapM1 significantly lowered blood glucose (34.2%), serum triglyceride (79.6%) and total cholesterol (62.1%) in type II diabetes induced rat. Therefore, the recombinant human gapM1 is successfully expressed in Pichia pastoris and effectively treated type II diabetes in rat models.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Pichia / Proteínas Recombinantes / Distribuição Aleatória / Ratos Sprague-Dawley / Usos Terapêuticos / Diabetes Mellitus Experimental / Tratamento Farmacológico / Adiponectina / Vetores Genéticos Limite: Animais Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2009 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Pichia / Proteínas Recombinantes / Distribuição Aleatória / Ratos Sprague-Dawley / Usos Terapêuticos / Diabetes Mellitus Experimental / Tratamento Farmacológico / Adiponectina / Vetores Genéticos Limite: Animais Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2009 Tipo de documento: Artigo