A novel screening system for Pseudomonas aeruginosa quorum sensing inhibitors / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1173-1179, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-296941
ABSTRACT
Quorum sensing is an important gene regulatory mechanism in Pseudomonas aeruginosa and controls the expression of numerous virulence factors. We designed and constructed a screening system for quorum-sensing inhibitors. We developed the system by using the lasI and rhlA promoters fused with promoterless sacB as reporters. Using this system we screened a number of Chinese herb extracts, and identified three herb extracts containing inhibitors to the quorum-sensing system and to its regulated genes. The screening system developed was highly efficient and sensitive. It could serve as a useful tool to identify herb compounds that block infections but unlikely render antibiotic resistance in pathogens.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Pseudomonas aeruginosa
/
Medicamentos de Ervas Chinesas
/
Química
/
Regiões Promotoras Genéticas
/
Genes Reporter
/
Acanthaceae
/
Valerianaceae
/
Avaliação Pré-Clínica de Medicamentos
/
Percepção de Quorum
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Estudo de rastreamento
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo
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