Microfluidic chip for detecting the expression of green fluorescent protein in Bacillus subtilis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1077-1081, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-296954
ABSTRACT
Laser scanning confocal microscope (LSCM) is currently the only equipment to observe fluorescence. However, this technique has disadvantages such as high cost and long test process. In this study, we developed a new system of laser-induced fluorescence (LIF) for microfluidic chip applied to detecting the expression of green fluorescent protein (GFP) in Bacillus subtilis. This novel system was comprised of laser device, optics unit, microfluidic chip, photomultiplier and computer treatment unit. The tests indicated that microfluidic chip could detect the expression of GFP as sensitively as LSCM in Bacillus subtilis. Moreover, this LIF detection system could instead of PCR to identify the positive clone in this special case. Nevertheless, the LIF system only was suitable to detect the fluorescent strength of GFP, and could not meet the request of some cases for example protein location. Therefore, this system will be applied in environmental detection with microbe, drug discovery and other cases.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bacillus subtilis
/
Proteínas de Fluorescência Verde
/
Técnicas Analíticas Microfluídicas
/
Genética
/
Metabolismo
/
Métodos
Idioma:
Inglês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS