Expression of interferon-λ1 in respiratory epithelial cells of children with RSV infection and its relationship with RSV load / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 677-681, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-297227
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of IFN-λ1 in respiratory epithelial cells of children with respiratory syncytial virus (RSV) infection and its relationship with RSV load.</p><p><b>METHODS</b>The nasopharyngeal swabs were collected from the children who were hospitalized with respiratory tract infection from June 2015 to June 2016. A direct immunofluorescence assay was used to detect the antigens of seven common respiratory viruses (including RSV) in the nasopharyngeal swabs. A total of 120 children who were only RSV positive were selected as the RSV infection group. A total of 50 children who had negative results in the detection of all viral antigens were selected as the healthy control group. Fluorescence quantitative real-time PCR was used to determine the RSV load and the expression of IFN-λ1 mRNA in the nasopharyngeal swabs of children in the two groups.</p><p><b>RESULTS</b>The expression of IFN-λ1 in the RSV infection group was significantly higher than that in the healthy control group (P<0.05). The expression of IFN-λ1 was positively correlated with RSV load (r=0.56, P<0.05).</p><p><b>CONCLUSIONS</b>RSV can induce the expression of IFN-λ1 in respiratory epithelial cells, suggesting that IFN-λ1 may play an important role in anti-RSV infection.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Virologia
/
Nasofaringe
/
Interleucinas
/
Infecções por Vírus Respiratório Sincicial
/
Carga Viral
/
Alergia e Imunologia
/
Células Epiteliais
/
Reação em Cadeia da Polimerase em Tempo Real
/
Microbiologia
Limite:
Criança, pré-escolar
/
Feminino
/
Humanos
/
Lactente
/
Masculino
/
Recém-Nascido
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2017
Tipo de documento:
Artigo
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