Establishment of a HPV-negative cell line derived from xenografted human laryngeal squamous cell carcinoma / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 533-536, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-297300
ABSTRACT
<p><b>OBJECTIVE</b>To establish a human laryngeal carcinoma cell line unassociated with human papillomavirus (HPV).</p><p><b>METHODS</b>Viable tissue of a well-differentiated laryngeal squamous cell carcinoma was obtained and tested negative for the presence of HPV by polymerase chain reaction. Minced tissue fragments were then transplanted subcutaneously into nude mice. After two successive passages, the tumor tissue was seeded into culture flasks and incubated in a medium containing 10% fetal bovine serum, insulin and epidermal growth factor. Tumor cell phenotype and molecular features were determined by various methods.</p><p><b>RESULTS</b>A stable cell line, designated as Lscc-02, was successfully established after 86 culture passages. The cells grew as a monolayer with epithelioid features. The cell doubling time was approximately 39.1 hours. The human origin of the tumor cells was confirmed by karyotype analysis. The squamous epithelial phenotype was demonstrated by the immunopositivity of anti-cytokeratin antibodies and ultrastructural presence of tonofilaments and desmosomes. The malignant nature of the cells was documented by their clonal formation in soft-agar and tumorigenicity in nude mice. Lscc-02 cells expressed carcinoembryonic antigen (CEA) and were negative for HPV DNA.</p><p><b>CONCLUSION</b>This newly established Lscc-02 laryngeal squamous cell carcinoma cell line may be a useful model for investigating laryngeal carcinoma unrelated to HPV infection, and the role of HPV in the progression of human laryngeal carcinoma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Papillomaviridae
/
Patologia
/
Virologia
/
Carcinoma de Células Escamosas
/
Neoplasias Laríngeas
/
Linhagem Celular Tumoral
/
Camundongos Endogâmicos BALB C
/
Camundongos Nus
/
Transplante de Neoplasias
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2005
Tipo de documento:
Artigo
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