Humoral immune response and in vitro neutralizing antibody assay on co-delivery of protein HPV16L1 virus-like particle with HPV16L1 gene / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 421-424, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-297332
ABSTRACT
<p><b>OBJECTIVE</b>To compare humoral immune response by co-inoculating mice with antigen HPV16L1 virus-like particle (VLP) and HPV16L1 recombinant plasmids and then observing the neutralizing antibody activity in vitro.</p><p><b>METHODS</b>C57BL/6 mice were injected intramuscularly/subcutaneously with pcDNA-L1 plasmids plus HPV16L1 VLP. Serum IgG levels were detected by ELISA, antibody neutralizing protective activities were determined by hemagglutination inhibition and HPV16L1 VLP binding inhibition assay.</p><p><b>RESULTS</b>Serum antibody titers and neutralizing antibody activities were increased in HPV16L1 plasmids plus HPV16L1 VLP proteins in co-immunized mice when compared with controls.</p><p><b>CONCLUSION</b>Co-inoculation of the HPV16L1 VLP protein can enhance production of neutralizing antibody activities against aimed antigen, which should be a more promising strategy for effective HPV16 prophylactic vaccine development.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Sangue
/
Proteínas Recombinantes
/
Imunoglobulina G
/
Testes de Neutralização
/
Células HeLa
/
Proteínas Oncogênicas Virais
/
Imunização
/
Proteínas do Capsídeo
/
Alergia e Imunologia
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2005
Tipo de documento:
Artigo
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