Effects of sodium arsenite on catalase in human keratinocytes / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 605-607, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-297634
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of sodium arsenite on the activity, the mRNA and the protein expression of CAT in human keratinocyte cell line (HaCaT).</p><p><b>METHODS</b>The activity of catalase (CAT) was detected by ultraviolet direct velocity assay. RT-PCR was used to detect the mRNA expression of CAT and Western blotting was conducted to detect the protein expression of CAT.</p><p><b>RESULTS</b>If the cells were treated with higher than 5.0 micromol/L sodium arsenite, the activity, mRNA and protein expression of CAT were decreased significantly and in a dosage dependent fashion (P < 0.05).</p><p><b>CONCLUSION</b>CAT is inhibited by sodium arsenite in the transcription, translation and activity levels.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Catalase
/
Queratinócitos
/
Linhagem Celular
/
Western Blotting
/
Compostos de Sódio
/
Arsenitos
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Relação Dose-Resposta a Droga
/
Toxicidade
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2006
Tipo de documento:
Artigo
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