Comparative analysis of proteins in seminal plasma of non-obstructive azoospermia patients and healthy fertile males / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyse protein alterations in the seminal plasma of non-obstructive azoospermia patients.</p><p><b>METHODS</b>Semen samples were collected from 11 healthy fertile and 6 azoospermia male volunteers respectively and tested by SELDI-TOF-MS with CM10 protein chip to get protein spectra maps, which were automatically treated with the special softwares of Ciphergen Inc.</p><p><b>RESULTS</b>The mean peak heights of 28 proteins expressed in the seminal plasma of the azoospermia patients were statistically different from those of the healthy fertile males (P < 0.05 ), of which 24 were of lower contents than in the normal controls, 4 with remarkably significant difference, M/Z 7 196.058, 7 630.573, 7 547.610 and 7 709.833 (P < 0.01).</p><p><b>CONCLUSION</b>The seminal plasma proteins of the azoospermia patients were significantly different from those of the healthy fertile males, with decreased contents of most of the different proteins, which might be significantly correlated with the development of azoospermia.</p>