Voltage-dependent anion channels (VDAC) on human sperm membrane / 中华男科学杂志
National Journal of Andrology
; (12): 498-501, 2007.
Article
em Zh
| WPRIM
| ID: wpr-297697
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To further study gene expression and characterization of voltage-dependent anion channels (VDACs) on human spermatozoa.</p><p><b>METHODS</b>VDACs were cloned by PCR from the testis cDNA library. Recombinant human sperm VDACs were produced in E. coli system by molecular cloning technology. Sperm membrane protein was extracted by 1% Triton X-100 and separated by chloroform/methanol.</p><p><b>RESULTS</b>The gene expression of VDACs was found in the human testis cDNA library and VDAC protein was detected located on the sperm membrane by alpha-helix.</p><p><b>CONCLUSION</b>VDAC proteins, abundant on the human sperm membrane and responsible for anion transportation, play an important role in sperm signaling transduction and fertility.</p>
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Índice:
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Assunto principal:
Fisiologia
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Espermatozoides
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Testículo
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Proteínas Recombinantes
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Transdução de Sinais
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Expressão Gênica
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Western Blotting
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Reação em Cadeia da Polimerase
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Canais de Ânion Dependentes de Voltagem
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Metabolismo
Limite:
Humans
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Male
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Article