Laser-assisted immobilization causes no direct damage to sperm DNA / 中华男科学杂志
National Journal of Andrology
;
(12): 216-218, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-297752
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether laser-assisted immobilization of sperm damages sperm DNA.</p><p><b>METHODS</b>Twenty-three semen samples were selected from an IVF program. Then normal spermatozoa were obtained by swimming-up method and immobilized with the tail by 0.45 ms pulse laser. Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and single cell gel electrophoresis (SCGE) were used to detect sperm DNA damage.</p><p><b>RESULTS</b>There was no significant difference either before and after laser treatment in the percentage of TUNEL-positive spermatozoa ([1.32 +/- 0.61]% vs [1.41 +/- 0.51]%, P > 0.05) or in SCGE ([1.59 +/- 0.70]% vs [1.83 +/- 0.68]%, P > 0.05).</p><p><b>CONCLUSION</b>Laser-assisted sperm immobilization may cause no direct damage to the sperm DNA.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Efeitos da Radiação
/
Sêmen
/
Motilidade dos Espermatozoides
/
Espermatozoides
/
Técnicas In Vitro
/
Dano ao DNA
/
Marcação In Situ das Extremidades Cortadas
/
Ensaio Cometa
/
Lasers
Tipo de estudo:
Estudo de etiologia
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Artigo
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