Genome sequencing and analysis of a peste des petits ruminants virus isolate, China/Tib/07 / 病毒学报
Chinese Journal of Virology
;
(6): 322-329, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-297863
ABSTRACT
Peste des petits ruminants virus is a member of Morbillivirus Paramyxoviridae. The complete genome of a Peste des petits ruminants virus (PPRV) isolate, China/Tib/07 was sequenced and molecular characteristics was analyzed. The internal sequences of the virus genome were amplified by RT-PCR with primers designed according to the published data in GenBank, while the sequences of the 3' and 5' ends of the genome were determined by RACE. Amplification products were directly sequenced,assembled and analyzed with DNAStar4.0. Results showed that China/Tib/07 genome consisted of 15 948 nucleotides in length, encoding six structural proteins and two non-structural proteins just like other known PPRV genomes. Phylogenetically, the virus genome shared homology of 91.6%-98.1% with Southwest Asian isolates among PPRV strains and the highest homology of 64.3% with rinderpest virus among morbillivirus members.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Doenças dos Ovinos
/
Proteínas Virais
/
Virologia
/
Células Vero
/
Ovinos
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Chlorocebus aethiops
/
China
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2010
Tipo de documento:
Artigo
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