Two recombinant adenovirus vaccine candidates containing neuraminidase Gene of H5N1 influenza virus (A/Anhui/1/2005) elicited effective cell-mediated immunity in mice / 病毒学报
Chinese Journal of Virology
; (6): 327-332, 2009.
Article
em Zh
| WPRIM
| ID: wpr-297953
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WPRO
ABSTRACT
The aim of this study is to develop the recombinant adenovirus vaccine (rAdV) candidates containing neuraminidase (NA) gene of H5N1 influenza virus and test in BALB/c mice the effect of cell-mediated immunity. In this study, two kind of NA gene (WtNA gene, the wild type; Mod. NA gene, the codon-modified type) derived from H5N1 influenza virus (A/Anhui/1/2005) were cloned and inserted respectively into plasmid of adenovirus vector, then the rAdV vaccines candidates (rAdV-WtNA and rAdV-Mod. NA) were developed and purified, followed by immunization intramuscularly (10(9) TCID50 per dose, double injection at 0 and 4th week) in BALB/c mice, the effect of cell-mediated immunity were analysed at 5th week. Results indicated that: (i) NA protein expression was detected in two rAdV vaccines candidates by Western blotting; (ii) the rAdV-Mod. NA vaccine could elicit more robust NA specific cell-mediated immunity in mice than that of rAdV-WtNA vaccine (P = 0. 016) by IFN-gamma ELIspot assay. These findings suggested rAdV-Mod. NA vaccine was a potential vaccine candidate against H5N1 influenza and worthy of further investigation.
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Índice:
WPRIM
Assunto principal:
Proteínas Virais
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Virologia
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Vacinas contra Influenza
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Distribuição Aleatória
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Western Blotting
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Reação em Cadeia da Polimerase
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Infecções por Orthomyxoviridae
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Alergia e Imunologia
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Virus da Influenza A Subtipo H5N1
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Genética
Tipo de estudo:
Clinical_trials
Limite:
Animals
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Female
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2009
Tipo de documento:
Article