Studies on the apoptosis of RK13 cells induced by rabbit hemorrhagic disease virus / 病毒学报
Chinese Journal of Virology
; (6): 316-317, 2009.
Article
em Zh
| WPRIM
| ID: wpr-297956
Biblioteca responsável:
WPRO
ABSTRACT
The apoptosis of RK13 cells induced by RHDV was investigated with DAPI staining, DNA ladder, Caspase 3 activity and flow cytometry, etc. The results showed that nuclear staining of infected cells with DAPI showed gradually morphological changes of the nuclei. As shown in the paper, a canonic oligonucleosome-sized DNA ladder was observed in cells harvested at 24h, 48h and 72h post-infection, confirming that DNA fragmentation was induced by RHDV infection. The results of flow cytometry showed that about 63% of cells were in apoptosis at 48h post-infection. Besides, we also demonstrated that the activation of Caspase 3 occurred during the infection process. In conclusion, our results showed that apoptosis in RHD might be determinant in the development of the pathogenesis of RHD.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fisiologia
/
Virologia
/
Linhagem Celular
/
Núcleo Celular
/
Apoptose
/
Vírus da Doença Hemorrágica de Coelhos
/
Infecções por Caliciviridae
/
Caspase 3
/
Fragmentação do DNA
/
Genética
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2009
Tipo de documento:
Article