Identification of radix et rhizoma clematidis and its adulterants using DNA barcoding / 药学学报
Acta Pharmaceutica Sinica
;
(12): 260-266, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-297982
ABSTRACT
This study provides the candidate sequences in the identification of Radix et Rhizoma Clematidis and its adulterants using DNA barcoding. We amplified and sequenced the region psbA-trnH, with the data of 284 sequences from GenBank, the differential intra- and inter-specific divergences, genetic distance, barcoding gap were used to evaluate five barcodes, and the identification efficiency was assessed using BLAST1 and Nearest Distance methods. The results showed that psbA-trnH barcodes performed high identification efficiency and inter-specific divergences among the five different DNA barcodes. Analysis of the barcoding gap and NJ tree showed psbA-trnH was superior to other barcodes. Based on the identification and PCR amplification efficiency, psbA-trnH can be the ideal barcode to identify Radix et Rhizoma Clematidis and its adulterants accurately.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Especificidade da Espécie
/
Contaminação de Medicamentos
/
Classificação
/
Raízes de Plantas
/
DNA de Plantas
/
Técnicas de Amplificação de Ácido Nucleico
/
Ranunculaceae
/
Rizoma
/
Código de Barras de DNA Taxonômico
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2014
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS