Antibody preparation and enzyme linked immunosorbent assay of Lonicera japonica phenylalnine ammonia lyase / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1498-1502, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-298052
ABSTRACT
The expression of phenylalnine ammonia lyase (LJPAL1) is closely related to the content of active compounds in Lonicera japonica. In this paper, a prokaryotic expression vector is constructed and LJPAL1 protein is expressed in E. coli. Three antigen sites were synthesized to peptide antigen and prepared polyclonal antibody of Anti-LJT-1, Anti-LJT-2 and Anti-LJT-3, separately. Antibody Anti-LJT-2 was screened using Western blotting. And indirect ELISA was built using Anti-LJT-2. The results of this study will be a base for honeysuckle chemical quality and evaluation kits.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenilalanina Amônia-Liase
/
Plantas Medicinais
/
Plasmídeos
/
Ensaio de Imunoadsorção Enzimática
/
Química
/
Western Blotting
/
Estrutura Terciária de Proteína
/
Estrutura Secundária de Proteína
/
Regulação da Expressão Gênica de Plantas
/
Lonicera
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2013
Tipo de documento:
Artigo
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