Exploration of the alternative splicing variants of rat phospholipase C-gamma 1 pre-mRNA / 南方医科大学学报
Journal of Southern Medical University
;
(12): 191-194, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-298208
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of phospholipase C-gamma 1 (PLC-gamma1) alternative splicing variants in rats.</p><p><b>METHODS</b>According to the sequence of human PLCG1 splicing variant, specific primers for rat PLC-gamma1 were designed and synthesized. The rat RNA was reverse transcribed into cDNA, which was amplified using the specific primers, and the PCR products were sequenced and analyzed using BLAST and bioinformatics methods. Totally 21 rat tissue samples were examined, including the heart, liver, lung, kidney, eyeball, and brain obtained in 3 different embryonic stages, 7 different early postnatal stages, and in adulthood.</p><p><b>RESULTS</b>The result did not show that rat PLC-gamma1 had the same splicing variant (PLC-gamma1a, NM_002660) as human does.</p><p><b>CONCLUSIONS</b>The same splicing variant of PLC-gamma1 detectable in human may not exist in rats, and the pre-mRNA may undergo splicing resulting predominantly in PLC-gamma1b mRNA. Very likely, the alternative splicing site of rat PLC-gamma1 is not identical to that of human.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Dados de Sequência Molecular
/
Precursores de RNA
/
Sequência de Bases
/
Análise de Sequência de DNA
/
Ratos Sprague-Dawley
/
Processamento Alternativo
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Sítios de Splice de RNA
/
Fosfolipase C gama
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS