Modification of vascular endothelial cell treatment for patch clamp study / 南方医科大学学报
Journal of Southern Medical University
;
(12): 175-180, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-298213
ABSTRACT
A modified protocol for quick and economic treatment of cultured human umbilical vein endothelial cells has been established, which result in high viability of the cells to allow better performance in patch-clamp studies. The electrophysiological properties of Ca (2+)-activated K(+) (KCa) channel of the cultured cells were investigated with a cell-attached configuration. With this modified treatment method, the cultured cells appear fusiform in shape under microscope, and KCa channel currents could be detected readily, suggesting their eligibility for patch-clamp studies.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Células Cultivadas
/
Reprodutibilidade dos Testes
/
Técnicas de Patch-Clamp
/
Biologia Celular
/
Canais de Potássio Cálcio-Ativados
/
Células Endoteliais
/
Potenciais da Membrana
/
Métodos
Tipo de estudo:
Guia de Prática Clínica
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Artigo
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