Differentially expressed transcription factor-related genes in nasopharyngeal carcinoma and nasopharyngeal tissues / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the gene expression profiles of transcription factor-related genes in nasopharyngeal carcinoma (NPC) tissues and normal nasopharyngeal tissues using a cDNA microarray membrane for exploring the regulatory mechanism of differential gene express in NPC tissues.</p><p><b>METHODS</b>The total RNAs from 24 NPC tissues and 24 pooled normal nasopharyngeal tissues were reverse transcribed and labeled with alpha-(32)P-dCTP. The resultant cDNAs were hybridized to GF211 microarray, and the signals were analyzed by Pathway 4.0 software. RT-PCR was carried out to confirm the results.</p><p><b>RESULTS</b>Among the 1625 differentially expressed genes detected in NPC and nasopharyngeal tissues, 35 transcription factor-related genes were identified with either up- or down-regulation.</p><p><b>CONCLUSION</b>These differentially expressed transcription factor-related genes in NPC tissues might play a role in the regulation of NPC-related gene expression.</p>