RP-HPLC determination of tiliroside in Daphne genkwa / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 301-303, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-298411
ABSTRACT
<p><b>OBJECTIVE</b>To determine the contents of tiliroside [kaempferol 3-O-beta-D-(6 " -p-coumaroyl) glucopyranoside] in the Daphne genkwa.</p><p><b>METHOD</b>The separation was performed on ZORBAX XDB C18 column (4.6 mm x 250 mm, 5 microm), using acetonitrile and water/acetic acid mixture (1,000/1) (gradient elution 0-18 min, 26 74; 18-33 min, 80 20) as mobile phase with the rate of 1.0 mL x min(-1) at 25 degrees C, the wavelength for measurement was 310 nm.</p><p><b>RESULT</b>The linearity was in the range of 0.1-5.0 microg (n=6), and the correlation coefficient was 0.9999. The average recovery for compound tiliroside was 101.50% (RSD 0.90%) (n=6).</p><p><b>CONCLUSION</b>This method is accurate, reliable and reproducible to determine tiliroside in the D. genkwa.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Acetonitrilas
/
Farmacologia
/
Flavonoides
/
Óleos Voláteis
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Caules de Planta
/
Daphne
/
Métodos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2009
Tipo de documento:
Artigo
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