Expression of tumor suppressor gene PRDM1 in lung cancers / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 434-436, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-298582
ABSTRACT
<p><b>OBJECTIVE</b>To determine the expression of tumor suppressor gene PRDM1 in lung cancers.</p><p><b>METHODS</b>Forty-five cases were enrolled in this study, including squamous cell carcinoma (20 cases), adenocarcinoma (15 cases), and small cell cancer (10 cases). PRDM1 protein was detected in paraffin-embedded tissue by immunohistochemistry. Tumor cells in lung cancers were further selected by laser microdissection for RT-PCR analysis. PRDM1 protein in frozen tissue was also detected by Western blot.</p><p><b>RESULTS</b>(1) PRDM1 protein was found in paraffin-embedded tissues in 90.0% (18/20) of squamous cell carcinoma, 13.3% (2/15) of adenocarcinoma, and 0 (0/10) small cell lung cancer. Squamous cell carcinoma predominantly expressed PRDM1 protein ( P < 0.01). (2) Gene product of PRDM1 DNA binding region was not found in microdissected tumor cells, but an abnormal PRDM1 protein about 70 KD was detected simultaneously in whole tumor tissue.</p><p><b>CONCLUSION</b>PRDM1 may be considered as a specific biomarker in pulmonary squamous cell carcinoma. The abnormal PRDM1 expression both at transcriptional and protein levels indicated that this tumor suppressor gene lost its function, which may become a new target in the strategy of treatment for lung cancers.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Repressoras
/
Fatores de Transcrição
/
Imuno-Histoquímica
/
Carcinoma de Células Escamosas
/
Adenocarcinoma
/
Biomarcadores Tumorais
/
Regulação Neoplásica da Expressão Gênica
/
Western Blotting
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Carcinoma de Células Pequenas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2007
Tipo de documento:
Artigo
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