Experimental study on apoptosis induced by aqueous extract of Taxus chinensis in human pulmonary carcinoma cell A549 and its molecular mechanisms / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 1243-1247, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-299030
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis inducing effects of aqueous extract of Taxus chinensis (AETC) in human lung cancer cell A549 and its molecular mechanisms.</p><p><b>METHODS</b>Using MTT assay, the inhibition of AETC on the proliferation of A549 cells was detected. The apoptosis was detected by light and electron microscopy, and Annexin V-FITC/PI staining. The expressions of ERK1/2, JNK1/2, and survivin were analyzed by Western blot.</p><p><b>RESULTS</b>AETC could inhibit the proliferation of A549 cells. Obvious apoptotic cells could be seen under light and electron microscope in AETC treated A549 cells. AETC's induction on the cell apoptosis was further confirmed by Annexin V-FITC/PI labeled quantitative detection. Western blot assay showed that AETC could decrease the survivin expressions. AETC showed no effects on ERK1/2 or JNK1/2 protein expressions.</p><p><b>CONCLUSION</b>AETC could significantly inhibit the proliferation of A549 cells and induce the apoptosis, which was mainly achieved through regulating the expressions of survivin.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Extratos Vegetais
/
Apoptose
/
Taxus
/
Sistema de Sinalização das MAP Quinases
/
Linhagem Celular Tumoral
/
MAP Quinases Reguladas por Sinal Extracelular
/
Proliferação de Células
/
Proteínas Inibidoras de Apoptose
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2011
Tipo de documento:
Artigo
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