Novel cationic liposome loading siRNA inhibits the expression of hepatitis B virus HBx gene / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1326-1330, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-299131
ABSTRACT
In order to solve the problem of selection and in vivo delivery problem in siRNA treatment, hepatitis B virus (HBV) HBx gene which could be targeted by siRNA was studied. The siRNA expression plasmid which specific inhibits HBx expression was obtained by in vitro selection via a dual-luciferase plasmid including HBx-Fluc fusion protein expression domain. The selected siRNA expression plasmid was then encapsulated in PEG-modified cationic liposome, which was devoted into pharmacodynamic studies at both cellular and animal level. The results illustrated that the cationic liposome which encapsulated siRNA expression plasmid could effectively inhibit HBx gene expression both in vitro and in vivo.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Cátions
/
Regulação Viral da Expressão Gênica
/
Transativadores
/
Química
/
Vírus da Hepatite B
/
RNA Interferente Pequeno
/
Genética
/
Lipossomos
/
Metabolismo
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2014
Tipo de documento:
Artigo
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