Experimental studies on male reproductive toxicity of bisphenol A in vitro and vivo / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 383-387, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-299220
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of Bisphenol A in adult rats and its possible mechanisms.</p><p><b>METHODS</b>BPA (in corn oil) was administered orally to 9-week-old male Sprague-Dawley rats for 14 days (0, 1 and 5 g/kg bw), and incubated primary Sertoli cells from pubertal SD rats with 0, 10(-7), 10(-6), 10(-5), 10(-4) mol/L BPA.</p><p><b>RESULTS</b>After oral administration, a significant decrease in right testis weight was observed in 5 g/kg dose group, but not in the 1 g/kg bw dose group. Germ cells were detached from basement membrane of seminiferous tubules and Sertoli cells in BPA-treated groups. Administration of BPA at 1 g/kg bw and 5 g/kg bw produced both nucleus pycnosis and vacuolized nucleus in germ cells and Sertoli cells. A marked loss in vimentin staining in Sertoli cells from testis of BPA-treated rats was detected. No change in levels of serum estradiol and testosterone was observed after two-week exposure to BPA. In Sertoli cell primary culture, BPA destroyed the cytoskeleton and cell-cell junctions, and elongated Sertoli cells.</p><p><b>CONCLUSION</b>These results suggest that BPA may injure reproductive function of male rats by destroying the cytoskeleton and changing the form of Sertoli cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tamanho do Órgão
/
Fenóis
/
Células de Sertoli
/
Testículo
/
Vimentina
/
Compostos Benzidrílicos
/
Citoesqueleto
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Biologia Celular
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2004
Tipo de documento:
Artigo
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