Induction of endothelial cell apoptosis by anti-alpha-enolase antibody / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 152-157, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-299394
ABSTRACT
<p><b>OBJECTIVE</b>To assess the prevalence of anti-alpha-enolase antibody in systemic autoimmune diseases in Chinese patients and its role in endothelial cell apoptosis.</p><p><b>METHODS</b>The reactivity of anti-alpha-enolase antibody in a variety of autoimmune disorders in Chinese patients was evaluated by dot blot assay. Endothelial cell apoptosis was investigated by in vitro incubation of endothelial cells with IgG purified from anti-alpha-enolase antibody-positive sera, with or without pre-incubation with recombinant alpha-enolase.</p><p><b>RESULTS</b>Anti-alpha-enolase antibody was prevalent in different systemic autoimmune diseases with relatively high reactivity in Chinese patients. In vitro incubation of endothelial cells with IgG containing anti-alpha-enolase antibody induced apoptosis in a time- and dose-dependent manner. Apoptosis was partly inhibited by pre-incubation of the endothelial cells with recombinant alpha-enolase.</p><p><b>CONCLUSIONS</b>Our data suggest that alpha-enolase is a common auto-antigen recognized by anti-endothelial cell antibodies in connective tissue disease. Interaction between alpha-enolase and its autoantibody plays a role in endothelial cell apoptosis. Changes other than cell killing may contribute to the pathogenesis of endothelial damage and microvascular lesions.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fosfopiruvato Hidratase
/
Autoanticorpos
/
Doenças Autoimunes
/
Immunoblotting
/
Linhagem Celular
/
Western Blotting
/
Apoptose
/
Biologia Celular
/
Células Endoteliais
Limite:
Adolescente
/
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Chinese Medical Sciences Journal
Ano de publicação:
2011
Tipo de documento:
Artigo
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