Protective effects of recombinant SCR15-18 domain of human soluble complement receptor type 1 on myocardial ischemia and reperfusion injury / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 1037-1040, 2007.
Article
em Zh
| WPRIM
| ID: wpr-299533
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of recombinant SCR15-18 domain of human soluble complement receptor type 1 (sCR1-SCR-15-18) in rats underwent myocardial ischemia and reperfusion (I/R).</p><p><b>METHODS</b>Sprague-Dawley rats were randomly divided into three groups (n = 12 each group): sham (SO); 30 min ischemia/3h reperfusion (I/R) and I/R plus sCR1-SCR15-18 (15 mg/kg before I/R, sCR1). Serum LDH, CK and cardiac myeloperoxidase (MPO) activity were measured. Infarct size, myocardial histopathological changes and myocardial C3c were also compared among groups.</p><p><b>RESULTS</b>Infarct size [(16.1 +/- 3.3)% vs. (22.9 +/- 3.0)%, infarct zone/left ventricular mass, P < 0. 05] and CK [(2532.5 +/- 597.1) U/L vs. (3400.9 +/- 534.9) U/L, P < 0. 05] and LDH [(5436.2 +/- 611.3) U/L vs. (6572.0 +/- 476.3) U/L, P < 0. 05] as well as MPO activity in infarct zone [(0.81 +/- 0.14) U/g vs. (1.12 +/- 0.13) U/g, P < 0.05] were significantly decreased post sCR1 compared to I/R group. sCR1 also significantly attenuated histological myocardial injury and reduced the deposition of C3c in infarct zone.</p><p><b>CONCLUSION</b>sCR1-SCR15-18 protein exerts cardioprotective effects in this rat I/R model.</p>
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Índice:
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Assunto principal:
Patologia
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Proteínas Recombinantes
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Receptores de Complemento
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Traumatismo por Reperfusão Miocárdica
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Receptores de Complemento 3b
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Ratos Sprague-Dawley
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Peroxidase
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Creatina Quinase
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Usos Terapêuticos
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Modelos Animais de Doenças
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2007
Tipo de documento:
Article